Amounts tested in animals
BPC-157 TB-500 is a mix of two small protein pieces.
This page gives the amounts tested in animals and how each was given. No controlled study has set a human dose for the blend.
What BPC-157 and TB-500 amounts did animal studies test?
BPC-157 TB-500 dosage doesn't come from a study of the blend. Some sellers label one vial as 10 milligrams of BPC-157 plus 10 milligrams of TB-500, or 20 milligrams in all [8].
Those numbers describe a package, not an amount proved to work. A label can't tell you what is safe or likely to help.
Micrograms are millionths of a gram, while nanograms are billionths. Animal studies tested each protein chain alone.
A rat tendon study used either 10 micrograms per kilogram or 10 nanograms per kilogram of BPC-157 [1]. Other rat work used about 10 micrograms per kilogram and 10 nanograms per kilogram.
Another rat stomach study used 400-800 nanograms per kilogram. Other animal tests used a wide spread of amounts for TB-500.
A rat stroke study tested 2-18 milligrams per kilogram of the full protein behind that short chain. Milligrams are larger units, and the amount near 3.75 milligrams per kilogram looked best.
Yet 18 milligrams per kilogram gave no benefit. One mouse study used 150 micrograms twice weekly for 6 months [6].
These animal facts aren't a plan for your body or injury. Higher amounts did not always work better, and no safe blend dose is known.
How fast does the body clear BPC-157 and TB-500?
Human studies have not shown how fast the body clears half of either protein chain. The timing for the blend is also unknown.
In rats and dogs, the body cleared half the BPC-157 in under 30 minutes. Human work tested full Thymosin Beta-4, not the short TB-500 chain [7].
Animal timing cannot set a schedule for people. It also cannot tell you how much time the pair remains in your body.
Do we know how fast the body clears BPC-157 and TB-500?
Human studies don't tell us how fast the body clears half of either protein piece. In rats and dogs, half the BPC-157 was gone in under 30 minutes.
Human work used full Thymosin Beta-4, not the short TB-500 piece [7]. The timing for you and the blend remains unknown.

How were the test substances given?
The phrase wolverine injection does not describe a tested human plan. Most animal studies injected a protein piece into the open space around the organs inside the belly [1][6].
Some tests put a shot below the skin; others used muscle. An early safety test, called Phase 1, used a vein but gave full Thymosin Beta-4.
Another small safety study put BPC-157 into a vein. Neither test proved a useful amount for the blend.
Some wound tests put one substance right on the hurt area. None of these methods shows how the blend would work in you.
Where a drug enters the body can change its effect. A shot into an animal's belly space cannot set a safe human shot.
Did BPC-157 and TB-500 animal timing show when to repeat a shot?
Human studies don't tell us how fast either protein piece leaves the body. In rats and dogs, half the BPC-157 was gone in under 30 minutes.
Human work with full Thymosin Beta-4 did not answer that question for the short TB-500 piece [7]. The animal result can't set shot timing for you.
What happened when a test substance was swallowed?
Animal studies gave BPC-157 by mouth because it can last in the stomach. Most claims about TB-500 come from shots of full Thymosin Beta-4, not the short piece [6][7].
No human study has tested BPC-157 with TB-500 by mouth. Sellers offer pills or liquids, but no study shows how much reaches a person's blood.
The published work cannot set a useful or safe swallowed amount for you. It also cannot show that the blend works when swallowed.
Can you trust what a vial label says?
Research laboratories may get both protein pieces as dry powder, then add germ-free water. A vial sold outside government drug rules may have the wrong contents, strength, or BPC-157:TB-500 amount.
In lab weight, the sold TB-500 piece is about 889 daltons, a unit for things too small to see. Full Thymosin Beta-4 weighs about 4963 daltons [7].
Those lab weights do not tell you a safe amount. They show that the short piece in a vial differs from the full protein used in much research.
Read how fast the body may clear each part and which long-term safety facts are missing. These pages do not give dose advice.